By Year By Month By Week Today Search Jump to month

Posttranslational Modifications in Bacteria-Host Interaction

Download as iCal file
x
Thursday, 30. June 2016 10:00 - 23:59

Dr. Feng Shao
Investigator and Deputy Director for Research, National Institute of Biological Sciences, Beijing, China
Howard Hughes Medical Institute International Early Career Scientist


Feng Shao authors 6 Nature, 2 Science and 2 Cell research papers (as corresponding author),
is an elected associate member of EMBO and a reviewing Editor of eLife

 

Many bacterial pathogens use a type III secretion system to inject virulent effector proteins into host
cells. These effectors often use sophisticated biochemical strategies to manipulate host signal
transduction pathway. I will present our work on four bacterial effector families, each of which
defines a novel enzymatic posttranslational modification, and their functions in infection. The OspF
effector from Shigella flexneri employs a MAPK phosphothreonine lyase activity to block host MAPK
signaling and IL-8 production. The CHBP/Cif family of effectors from Burkholderia pseudomallei and
enteropathogenic E. coli (EPEC) catalyze deamidation of Gln-40 in ubiquitin and NEDD8, thereby
inactivating host ubiquitin pathway and many related cellular processes. NleE from EPEC and
Salmonella typhimurium carries out a novel cysteine methylation modification on key ubiquitin-chain
sensory proteins in host NF-KB pathway, resulting in blocking of NF-KB-mediated inflammatory
response during infection. The NleB effector targets host death domain-containing proteins in the
death receptor pathway for arginine GlcNAcylation and this unique modification is critical for EPEC
replication in the colons of infected mice.
Recently, we have also identified a family of iron-containing dodecameric bacterial autotransporter
heptosyltransferase (BAHT) family. BAHT-catalyzed autotransporter hyper-heptosylation mediates
Escherichia coli adhesion to host cells and is essential for Citrobacter rodentium colonization in mice.
These findings reveal unexpected enzymatic space in bacterial virulence mechanism and highlight the
importance of posttranslational modifications in bacteria-host interactions.

Host: Alla Zamyatina

Location : Seminar Room 03/03 Department of Chemistry, BOKU II, Muthgasse 18, Wien
Contact : VIBT/ÖGMBT-Seminar

Back