MASTER THESIS "DEVELOPMENT OF A COLORIMETRIC DETECTION SYSTEM FOR BIOGENIC AMINES"
Standort: Tulln (in immediate vicinity of Vienna) AIT Austrian Institute of Technology GmbH
Art: Masterarbeit
Firma: AIT Austrian Institute of Technology GmbH
Eingetragen am: 2019-01-16
Kontakt:
Beschreibung:

We are Austria’s largest Research and Technology Organisation and an international player in the research areas that we cover. This makes us a leading development partner for industry and a top employer in the scientific community. Our Center for Health & Bioresources in Tulln (in immediate vicinity of Vienna) invites applications for a:

 

Master Thesis "Development of a colorimetric detection system for biogenic amines"

 

IMAGINE

 

  • The consumption of food containing biogenic amines is reported to be responsible for many pharmacological effects (diarrhea, headache, asthma, hypotension) which lead to several types of foodborne disease, including histamine poisoning, mainly from ingestion of certain species of fish (scombroid poisoning), and tyramine toxicity (cheese reaction).

 

ENGAGE

 

  • The master student will work on the proof-of-concept of such a colorimetric detection system using commercial diamine oxidase. Diamine oxidase (DAO) catalysis the degradation of biogenic amines under consumption of oxygen and formation of hydrogen peroxide and ammonia. Hydrogen peroxide can be detected by either horseradish peroxidase (HRP) and tetramethylbenzidine (TMB) as substrate or metal nanoparticles, e.g. Ag nanoparticles. Commercial nanoparticles will be tested for this purpose as well as nanoparticles biosynthesized in house using plant extracts. Their size and shape will be determined using dynamic light scattering and microscopic techniques. Alternatively, degradation of biogenic amines might be measured via decrease in oxygen.
  • After a solid literature review the master student will develop a detection system for hydrogen peroxide and alternatively for oxygen and demonstrate its feasibility for detection of putrescine and histamine as two important examples of biogenic amines. Once the detection system works in solution transfer to a solid state system is considered.

 

ACHIEVE

 

  • We will develop a multiplex diagnostic tool that is capable of reliably and simultaneously measuring histamine, tyramine, putrescine and other biogenic amines with the overall goal to provide a validated detection system for further investigations.

 

Your qualifications as an Ingenious Partner (m/f):

  • Profound expertise in (bio)chemistry or related fields
  • Good skills in English
  • Interest to work in a multidisciplinary team


What to expect:
EUR 719,- gross per month for 20 hours/week based on the collective agreement. There will be additional company benefits. As a research institution, we are familiar with the supervision and execution of Master theses and we are looking forward to supporting you accordingly!

 

TOMORROW TODAY –WITH YOU?

Then submit your application documents (CV, motivational letter, certificates) via https://jobs.ait.ac.at/Job/102267

Anhang: hier